Construction of scrA " " l a c Z gene fusions to investigate regulation of the sucrose PTS of S
نویسندگان
چکیده
The scrA gene coding for sucrose EnzymelI of the phosphoenolpyruvate dependent phosphotransferase system previously isolated from Streptococcus mutans was fused in vitro to the promoterless lacZ" gene to monitor the expression of the scrA gene. The scrA :: lacZ gene fusion was introduced back into S. mutans GS-5IS3 by two independent transformation procedures involving either linear or plasmid DNA to produce both scrA and scrA + mutants. These mutants should prove useful for analyzing the regulation of sucrose transport in S. rnutans.
منابع مشابه
Construction of homologous and heterologous synthetic sucrose utilizing modules and their application for carotenoid production in recombinant Escherichia coli.
Sucrose is one of the most promising carbon sources for industrial fermentation. We expressed synthetic modules expressing genes of the PEP-PTS and non-PTS pathways in Escherichia coli K12 for comparison. We selected PEP-PTS pathway genes of Lactobacillus plantarum and Staphylococcus xylosus and non-PTS pathway genes of sucrose-utilizing (Scr(+)) E. coli EC3132. Switchable Scr(+) modules expres...
متن کاملAnalysis of a Catabolic Operon for Sucrose Transport and Metabolism in Clostridium acetobutylicum ATCC
The utilization of sucrose by Clostridium acetobutylicum ATCC 824 was investigated. Sucrose was found to be transported via a phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) and a metabolic pathway identical to that previously identified in C. beijerinckii, was established. The genes encoding the proteins of this pathway were identified from the C. acetobutylicum genome sequ...
متن کاملComprehensive mutational analysis of sucrose-metabolizing pathways in Streptococcus mutans reveals novel roles for the sucrose phosphotransferase system permease.
Sucrose is perhaps the most efficient carbohydrate for the promotion of dental caries in humans, and the primary caries pathogen Streptococcus mutans encodes multiple enzymes involved in the metabolism of this disaccharide. Here, we engineered a series of mutants lacking individual or combinations of sucrolytic pathways to understand the control of sucrose catabolism and to determine whether as...
متن کاملRegulation of sucrose-6-phosphate hydrolase activity in Streptococcus mutans: characterization of the scrR gene.
Previous results have implicated an important role for the enzyme IIScr, the sucrose-specific permease, in the transport of sucrose by cariogenic Streptococcus mutans. The product of the scrB gene, sucrose-6-phosphate hydrolase (Suc-6PH), is required for the metabolism of phosphorylated sucrose. The results from the utilization of scrB::lacZ fusions in S. mutans GS-5 have suggested that sucrose...
متن کاملMicroarray analysis of gene expression patterns in Arabidopsis seedlings under trehalose, sucrose and sorbitol treatment
Trehalose is the non-reducing alpha-alpha-1, 1-linked glucose disaccharide. The biosynthesisprecursor of trehalose, trehalose-6-phosphate (T6P), is essential for plant development, growth,carbon utilization and alters photosynthetic capacity but its mode of action is not understood. In thecurrent research, 6 days old seedlings of Arabidopsis thaliana (Columbia ecotype) were grown inliquid cultu...
متن کامل